Cell Line Description

DerivationPrimary cell type: Fibroblast of dermisTissue collection date: Aug. 2, 2012Passage number reprogrammed: 3Reprogramming methodVector type: IntegratingVector: VirusVirus type: RetrovirusGene list: KLF4MYCNANOGPOU5F1SOX2Absence of reprogramming vector(s): yesHave the reprogramming vectors been silenced: YesMerthods used: rtpcrOther:From the same donor: UOXFi008-B, UOXFi008-CCharacterizationAnalysis of Undifferentiated CellsMarker expression: NANOG (+)TRA 1-60(+)PluriTest: Pluripotency score: 33.82 Novelty score: 1.33 Report: MK144-1.PNGMicrobiology / Virus ScreeningHIV 1: -HIV 2: -Hepatitis B: -Hepatitis C: -Mycoplasma: NegativeGenotypingKaryotypingPassage number: 17Cell line karyotype: 46, XXKaryotyping method: SNP analysis Illumina Human CytoSNP-12v2.1 bead chip array

Cell Line Origin

DepositorUniversity of Oxford

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

EBiSC is a trademark of Fraunhofer-Gesellschaft

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation

Linkage

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Surface coating: VitronectinPassage method: Enzyme-free cell dissociation / EDTAO₂ concentration: 21%CO₂ concentration: 5%Culture medium: Essential E8^®Passage Number banked (pre-EBiSC): 6