General description

A cell-permeable acetohydrazide that dose-dependently inhibits hypoxia-induced mitochondrial ROS generation (≤10 µM) in HepG2 and other cells, and suppresses cellular growth (≤50 µM) in HUVEC, HeLa, and HCT15 cultures. At ≤10 µM, this compound strongly inhibits VGFR-induced tube formation and invasive activity in HUVECs in vitro without obvious toxicity. It is also shown to hinder capillary formation in a chick embryo CAM assay without any sign of thrombosis and hemorrhage in vivo. Furthermore, it attenuates the expression levels HIF-1α and its target gene VEGF (0–10 µM) dose-dependently.

A cell-permeable acetohydrazide that dose-dependently inhibits hypoxia-induced mitochondrial ROS generation (≤ 10 µM) in HepG2 and other cells, and suppresses cellular growth (≤ 50 µM) in HUVEC, HeLa, and HCT15 cultures. At ≤ 10 µM, this compound strongly inhibits VGFR-induced tube formation and invasive activity in HUVECs in vitro without obvious toxicity. It is also shown to hinder capillary formation in a chick embryo CAM assay without any sign of thrombosis and hemorrhage in vivo. Furthermore, it attenuates the expression levels HIF-1α and its target gene VEGF (0–10 µM). dose-dependently.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kim, K.H., et al. 2011. Biochem. Biophys. Res. Commun.404, 541.

Packaging

5 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)