General description

Non–ionic detergent intended for solubilizing membrane–bound proteins in their native state and for the preparation of lipid vesicles. Its well–defined chemical structure, small uniform micelles, and high water solubility make it superior to most other nonionic detergents for membrane solubilization. Because of its high critical micelle concentration (20-25 mM), it has the additional advantage of being removed easily by dialysis when compared to bile salts. Aggregation number: 84.

Absorbance (10%, H₂O, 260 nm):≤0.3.

Nonionic detergent intended for solubilizing membrane-bound proteins in their native state and for the preparation of lipid vesicles. Its well-defined chemical structure, small uniform micelles, and high water solubility make it superior to most other nonionic detergents for membrane solubilization. Because of its high critical micelle concentration (20-25 mM), it has the additional advantage of being removed easily by dialysis when compared to bile salts. Aggregation number: 84.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hoshino, T., et al. 1992. J. Biol. Chem.267, 21313.Levy, D., et al. 1992. Biochim. Biophys. Acta1107, 283.Nunn, R.S., et al. 1992. J. Mol. Biol.228, 1259.Lorber, B., et al. 1990. Biochim. Biophys. Acta1023, 254.Jopski, B., et al. 1989. Biochim. Biophys. Acta978, 79.Camm, E.L., et al. 1981. Plant Physiol.67, 1061.Gould, R.J., et al. 1981. Biochemistry20, 6776.

Packaging

25, 100 g in Glass bottle

1, 5 g in Plastic ampoule

Reconstitution

Following reconstitution, store in the refrigerator (4°C), under sterile conditions. Aqueous stock solutions are stable for up to 3 months at 4°C. Solutions are susceptible to hydrolysis (especially acidic solutions) and enzymatic hydrolysis.

Warning

Toxicity: Standard Handling (A)