Ethidium bromide solution, for fluorescence, approx. 1% in H2O

Code: 46067-50ML-F D2-231

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon bind...


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£89.20 50ML
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Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 µg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 µg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 µg/ml and visualized immediately after electrophoresis.

concentration~1% in H2O
fluorescenceλex 530 nm; λem 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA), λex 480 nm; λem 620 nm in H2O, λex 518 nm; λem 605 nm (bound to DNA)
gradefor fluorescence
InChI keyZMMJGEGLRURXTF-UHFFFAOYSA-N
InChI1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
Quality Level100
SMILES string[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
storage temp.2-8°C
Code
Description
Unit Size
List Price
Qty
46067-250ML-F
Unit:250ML
List Price: £357.00
Source:List Price
ADD
Cas Number1239-45-8
Hazard Class6.1
Un Number2810
Pack GroupIII
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