Application

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Esterase from porcine liver has been used as a negative control in fluorescence measurement studies.

Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.

Porcine liver esterase (PLE) displays good stability, broad substrate specificity, and is a low-cost enzyme. It is useful in the hydrolysis of ester- and amide-containing compounds, to free acids and is useful detoxification of xenobiotics.

Components

contains 1,4-dithioerythritol

General description

Porcine liver esterase (PLE) is localized in the endoplasmic reticulum (ER).

Other Notes

Sales restrictions may apply

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol ethyl valerate (CAt. No. 30784) per minute at pH 8.0 and 25°C