Biochem/physiol Actions
Reversible: no
Product does not compete with ATP.
Target IC50: 8 µM, 10 µM, 300 nM, 200 nM against cyclooxygenase, 5-lipoxygenase, 12-lipoxygenase, and 15-lipoxygenase , respectively, in whole cells
Primary TargetPLA2
Cell permeable: yes
General description
Cell-permeable. Inhibits arachidonic acid uptake and arachidonic acid specific and nonspecific acyl-CoA synthesis. Inhibits phospholipase A2 (PLA2) and cytochrome P-450. Inhibits cyclooxygenase (ID50 = 8 µM), 5-lipoxygenase (ID50 = 10 µM), 12-lipoxygenase (ID50 = 300 nM), and 15-lipoxygenase (ID50 = 200 nM) in whole cells. A potent modulator of Ca2+ entry into cells. Stimulates luteinizing hormone release from cultured pituitary cells.
Cell permeable. Inhibits arachidonic acid uptake and the activities of arachidonic acid specific and non-specific acyl-CoA synthetases. Inhibits phospholipase A2 (PLA2) and cytochrome P-450. Inhibits cyclooxygenase (ID50 = 8 µM), 5-lipoxygenase (ID50 = 10 µM), 12-lipoxygenase (ID50 = 300 nM), and 15-lipoxygenase (ID50 = 200 nM) in whole cells. Also acts as a potent modulator of Ca2+ entry into cells. Blocks monocyte binding in minimally-oxidized LDL in endothelial cells. Stimulates luteinizing hormone release from cultured pituitary cells.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Honda, H.M., et al. 1999. Arterioscler. Thromb. Vasc. Biol.19, 680.Ekokoski, E., and Tornquist, K. 1994. Biochim. Biophys. Acta 1223, 274.Ondrey, F., et al. 1989. Cancer Res.49, 1138.Kuhn, H., et al. 1984. Eur. J. Biochem.139, 577.
Packaging
20 mg in Plastic ampoule
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria: