Biochem/physiol Actions

Cell permeable: no

Primary TargetBlocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus

Reversible: yes

Product does not compete with ATP.

General description

Specifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at ﹤40 ng/ml. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange.

Specifically and reversibly blocks translocation of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus without affecting endocytosis or lysosome function. Inhibitor of HDL-mediated cholesterol efflux. Causes disassembly of the Golgi complex and ER swelling in a variety of mammalian cell lines at ﹤40 ng/ml. Blocks binding of ADP-ribosylation factor to the Golgi and inhibits the GDP-GTP exchange. Inhibits the activity of BIG1 and BIG2, the guanine-nucleotide exchange proteins for ADP-ribosylation factors. Also available as a 25 mM solution in DMSO (Cat. No. 500583).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Yamagi, R., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 2567.Moriango, N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 12856.Mendez, A.J. 1995. J. Biol. Chem. 270, 5891.Ridgway, N.D., et al. 1995. J. Biol. Chem. 270, 8023.Tsai, S.C., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3063.Ktistakis, N.Y., et al. 1992. Nature356, 344.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 2 months at -20°C.

Warning

Toxicity: Harmful (C)