Biochem/physiol Actions
Cell permeable: no
Reversible: no
Product does not compete with ATP.
General description
The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity toward ATPase (IC50 ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).
The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity towards ATPase (IC50 = ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC50 ~3 µM).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Eddinger, T.J. et al. 2007. J. Pharmacol. Exp. Ther.320, 865.Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.Straight, A.F., et al. 2003. Science299, 1743.
Packaging
1 mg in Plastic ampoule
Packaged under inert gas
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month in 90% DMSO or methanol (-20°C). Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.
Warning
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
This product has met the following criteria: