Analysis Note

homogeneous single spot by TLC

General description

Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.

Packaging

50 mg in Plastic ampoule

Reconstitution

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Warning

Toxicity: Standard Handling (A)