Biochem/physiol Actions

Target Sub-Family: STE

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

GCN2 (EIF2AK4) belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1) to downregulate protein synthesis in response to varied cellular stresses.

Product Source: N-terminal GST-tagged, recombinant, human GCN2 full length, expressed by baculovirus in Sf21 insect cells. The recombinant protein contains the amino acid substitution E556G with reference to GenPept NP_001013725. E556G is reported in GenPept CAH10626.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size as catalog # 14-934M and in bulk as catalog # 14-934-K.

Physical form

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Purified using glutathione sepharose.

Quality

Routinely evaluated by phosphorylation of peptide RSRSRSRSRSRSRSR

Storage and Stability

6 months at -70°C