Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %Identity (IR-spectrum): passes testAppearance: Colorless crystals or white crystalline powder.Appearance of solution (100 g/l, water): Clear and colorless.Melting point (DSC (differential scanning calorimetry)): 132 - 135 °CUV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1Cyanide (CN): ≤ 0.000001 %Chloride (Cl): ≤ 0.0005 %Sulfate (SO₄): ≤ 0.001 %Heavy metals (as Pb): ≤ 0.0004 þ (Iron): ≤ 0.00002 %NH₄ (Ammonium): ≤ 0.00001 %Biuret: ≤ 0.05 %Sulfated ash (800 °C): ≤ 0.01 %

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Packaging

5 kg in Plastic bottle