p30,

Application

p30 is an endogenous truncated form of p39 that associates with Cdk5 to form Nclk, which regulates neurotransmission, axon guidance, central nervous system (CNS) architecture, and pathogenesis. Therefore, p30 is useful in studying neurodegenerative diseases such as Alzheimer’;s, Amyotrophic Lateral Sclerosis, and Parkinson’;s Disease.

Biochem/physiol Actions

P30 activates GST-Neuronal cyclin-dependent kinase 5 (Cdk5). It also associates with Cdk5 to form an activated neuronal Cdc2-like kinase (Nclk). Product P1246 is recombinant, from bovine and is expressed in E. coli.

Physical form

p30 is supplied in a solution of 25 mM Tris, pH 7.4, 1 mM DTT, and 30% glycerol. (0.5 µg protein/ 25 ul vial)

Preparation Note

Expressed as a GST fusion protein.

Unit Definition

One unit will incorporate 1 picomole of phosphate into histone H1 peptide per minute at pH 7.2 at 30 °C.