Application

Mps1-IN-3 has been used in 2D-proliferation and 3D-organotypic culture outgrowth assays.

Biochem/physiol Actions

Mps1 mediates phosphorylation of kinetochore scaffold 1.

Mps1-IN-3 is an inhibitor of Monopolar spindle 1 (MPS1, also known as TTK), a Dual-specificity kinase required for spindle pole body duplication and spindle checkpoint function. Mps1-IN-3 inhibited Mps1 kinase activity with an IC₅₀ value of 50 nM inhibited the proliferation of U251 glioblastoma cells as determined by cell counting with an IC₅₀ value of approximately 5 µM, and sensitized glioblastoma cells to vincristine in orthotopic mouse models.

General description

Monopolar spindle 1 (Mps1) protein contains a TPR (tetratricopeptide repeat) motif at its N-terminal and a C-terminal kinase domain.

Packaging

5, 25 mg in glass bottle