Analysis Note
Identity (UV/VIS-Spectrum): passes testAbsorption maximum λmax. (buffer pH 7.0): 577 - 584 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570TLC-Test: passes testLoss on drying (110 °C): ≤ 8 %Suitability for electrophoresis: passes test
Application
Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.
General description
Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.
Packaging
25 g in Glass bottle
This product has met the following criteria: