Analysis Note

ControlHeLa cell lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Western Blot Analysis: 0.1 µg/mL from a representative lot detected TFG on 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFG in mouse hippocampus, mouse cerebellum, human placenta, and human pancreas tissues.

This Anti-TFG Antibody is validated for use in Western Blotting, IHC(P) for the detection of TFG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The TRK-fused gene protein (TFG) gene is highly conserved among mammalian species, and is widely studied as a fusion gene partnering with various oncogenes. The function of the resultant TFG protein product is still unclear. TFG regulates the activity of phosphotyrosine-specific phosphatase-1 (SHP-1), and may be important in cells containing SHP-1 such as epithelial and neuronal cells. Defective TFG contributes to thyroid papillary carcinoma.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human TFG.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected TFG on 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes the C-terminus of TFG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~56 kDa observed. The calculated molecular weight is 43 kDa, however TFG has been shown as a ~56 kDa band in western blots (Greco, A., et al. (1995). Molecular and Cellular Biology. 15(11):6118–6127.)