Anti-N-cadherin, CT, clone 6A9.2,AF488

Application

This mouse monoclonal Anti-N-cadherin, CT Antibody, clone 6A9.2, Alexa Fluor^® 488 Conjugate, Cat. No. MABT530-AF488 is tested in Immunocytochemistry for the detection of N-Cadherin.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Cadherin in HeLa cells.

The unconjugated antibody (Cat. No. MABT530) is shown to be suitable also for Western blotting and Immunohistochemistry (Paraffin) applications.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.

Immunogen

GST/His-tagged recombinant fragment corresponding to 79 amino acids from the N-terminal half of human cadherin-2.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS with 1.5% BSA and 0.05% sodium azide.

Protein G purified

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected N-Cadherin in A431 cells.

Specificity

Clone 6A9.2 specifically detects N-cadherin extracellular cadherin 1 domain. It targets an epitope with in a sequence of 79 amino acids in the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

99.81 kDa calculated.