Analysis Note

Positive ControlHuman or rat brain tissues

Application

ELISA (0.1-0.5 µg/ml)r>Immunoblotting (0.1-0.5 µg/ml)r>Frozen Sections (10-50 µg/ml)r>Immunoprecipitation (2-5 µg/ml)r>Paraffin Sections (10-50 µg/ml, see comments)

General description

Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).

Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.

This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

Immunogen

bovine GFAP

Bovine Brain

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Tohyama, T., et al. 1993. Am. J. Pathol.142, 871.Lee, V.M., et al. 1984. J. Neurochem. 42, 25.

Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)