Analysis Note

ControlRat liver mitochondria tissue lysate

Application

Anti-CPT2 detects levels of CPT2 proteins & has been published & validated for use in WB.

General description

Carnitine palmitoyltransferase II (CPT2, or CPT II) belongs to the carnitine/choline acetyltransferase family. It is a ubiquitous protein found in the inner membrane of the mitochondria and plays an essential role in fatty acid β-oxidation. CPT2 is part of the system involving CPT1 and carnitine/acylcarnitine translocase (CACT), that facilitate the transport of long chain fatty acids into the mitochondrial matrix. CPT2 plays the role of restoring these molecules to original esterified forms suitable for β-oxidation, after they arrive in the matrix. Defects in CPT2 has varying outcomes: in adults, it results in episodes of rhabdomyolysis (breakdown of muscle fibers which circulate in the bloodstream), after prolonged and vigorous exercise; and in infants, CPT2 deficiency induces hypoketotic hypoglycemia and eventual cardiac damage.

Immunogen

KLH-conjugated linear peptide corresponding to human CPT2.

Quality

Evaluated by Western Blot in rat liver mitochondria tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected CPT2 on 10 µg of rat liver mitochondria tissue lysate.

Target description

~68 kDa observed (cleaved form). Uniprot gives a calculated weight of 74 kDa; however, CPT2 has been observed at ~68 kDa (Faye, A, et al. (2007). The Journal of Biological Chemistry. 282(37):26908–26916.). Uncharacterized bands appear at ~55, 58, 72, and 100 kDa in some lysates.