Application

Research CategoryNeuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Calretinin in rat brain tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calretinin in human cerebral cortex and rat cerebellum tissue sections.

Anti-Calretinin Antibody, Cat. No. ABN2191, is a highly specific rabbit polyclonal antibody that targets Calretinin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has multiple calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7 20 µm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070).

Immunogen

GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin.

Epitope: domain of:

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in human fetal brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Calretinin in human fetal brain tissue lysate.

Specificity

This rabbit polyclonal antibody detects Calretinin in human and rat cells. It targets an epitope within 99 amino acids from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~31 kDa observed; 31.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).