Anti-PHOX2B

Analysis Note

ControlHEK293 cell lysate

Application

Detect Phox2a using this rabbit polyclonal antibody, Anti-PHOX2B Antibody validated for use in western blotting.

Research CategoryStem Cell Research

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Paired mesoderm homeobox protein 2B (PHOX2B), also known as Neuroblastoma Phox (NBPhox), PHOX2B homeodomain protein, and Paired-like homeobox 2B is a paired-homeodomain transcription factor that maintains the noradrenergic phenotype during embryogenesis. PHOX2 proteins help regulate endogenous hydroxylases in neural crest cells and aid in sympathetic neuron generation. PHOX2A controls only the differentiation of the main noradrenergic center of the human brain, while PHOX2B is required for the differentiation of all central and nonperipheral noradrenergic centers. PHOX2B is expressed in neuroblastoma, brain and adrenal gland. PHOX2B defects can cause congenital central hypoventilation syndrome (CCHS) and susceptibility to neuroblastoma type 2 (NBLST2). PHOX2 regulation may have therapeutic potential in aging or degenerative noradrenergic neuron disorders.

Immunogen

KLH-conjugated linear peptide corresponding to human PHOX2B.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PHOX2B in 15 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed. Uncharacterized bands may appear in some lysate(s).