Analysis Note

ControlDaudi cell lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected JPH3 in human brain tissue.
Immunofluorescence Analysis: 20 µg/mL from a representative lot detected JPH3 in human brain cells.

Anti-JPH3 Antibody is a highly specific rabbit polyclonal antibody, that targets Junctophilin-3 & has been tested in western blotting, IHC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Junctophilin-3 (JPH3, JP-3), also known as Trinucleotide repeat-containing gene 22 protein (TNRC22), is a single-pass type IV membrane protein belonging to the junctophilin family. Junctophilins are important components of the junctional complexes which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. JPH3 is specifically expressed in brain. A mutation associated with JPH3 is the cause of Huntington disease-like type 2 (HDL2), a neurodegenerative disorder.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence near the C-terminus of human JPH3.

Epitope: Near C-terminus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal antibody in buffer containing PBS with 0.02% sodium azide.

Affinity purified.

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in Daudi cell lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected JPH3 in 15 µg of Daudi cell lysate.

Specificity

This antibody recognizes the C-terminus of JPH3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~105 kDa observed. Uniprot describes 2 isoforms at ~20 kDa and ~81 kDa Uncharacterized bands may appear in some lysates.