HISTRAP FF 5 X 1 ML

Code: GE17-5319-01 D2-232

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• High binding cap...


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Your Price
£176.00 PK5
£211.20 inc. VAT

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• High binding capacity, approx. 40 mg/mL medium.• Negligible leakage of Ni2+.• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

General description

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).Ni Sepharose® 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.The high flow rates made possible by the Ni Sepharose® 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Legal Information

HiTrap is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

HisTrap is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

average diameter90 µm
bed volume5 mL
mfr. no.GE Healthcare, 17-5319-01
column I.D.16 mm
particle size45-165 µm
bed size16 mm × 25 mm
capacity~40 mg binding capacity(histidine-tagged protein)
packagingpack of 5 × 1 mL
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
cleaning2 - 14 (Ni2+-stripped medium.)
working range3 - 12 (Ni2+-stripped medium.)
parameter≤20 mL/min flow rate
parameter42 psi
matrix6% cross-linked agarose
suitabilitysuitable for bioprocess medium
Cas NumberMixture
This product has met the following criteria: