Analysis Note
To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.
Features and Benefits
• High binding capacity, approx. 40 mg/mL medium.• Negligible leakage of Ni2+.• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
General description
HisTrap™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).Ni Sepharose® 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.The high flow rates made possible by the Ni Sepharose® 6 Fast Flow matrix make HisTrap™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.
Legal Information
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
HisTrap is a trademark of GE Healthcare
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
This product has met the following criteria: