Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• High binding capacity, approx. 40 mg/mL medium.• Negligible leakage of Ni²⁺.• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

General description

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^® 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).Ni Sepharose^® 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.The high flow rates made possible by the Ni Sepharose^® 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Legal Information

HiTrap is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

HisTrap is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)