Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

Designed for scaling up purification of histidine-tagged proteins High protein binding capacity and minimal leakage of Ni2? ions As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support. Available in convenient prepacked HisPrep^™ and HisTrap^™ column formats.

General description

Ni Sepharose^® 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose^® Fast Flow platform.Ni Sepharose^® 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.Ni Sepharose^® 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity..Ni Sepharose^® 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose^® 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Legal Information

HisPrep is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose productsThese products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HisTrap is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)