Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^® 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap^™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Features and Benefits

Convenient, prepacked gravity-fLow columns. Allows direct purification of both clarified and unclarified cell lysates. Yields up to 20 mg of pure Proteins per column. Yields up to 20 mg of pure Proteins per column

General description

GST GraviTrap^™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose^® 4B, sufficient for purification of up to 20 mg of fusion protein.

Legal Information

GraviTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)