Analysis Note

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Application

IMAC Sepharose^® 6 Fast FLow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.IMAC Sepharose^® 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose^® 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC Sepharose^® 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, IMAC Sepharose^® 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support

Features and Benefits

• Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity• Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion• As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.• Available in the convenient and time-saving prepacked HiTrap^® and HiPrep^™ formats.

General description

IMAC Sepharose^® 6 Fast FLow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose productsThese products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

HiTrap is a registered trademark of GE Healthcare

HiPrep is a trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)