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ROCHE Interleukin-2 human (hIL-2)recombinant (E. coli)

Code: 11147528001 D2-231

Application

Recombinant IL-2, human, allows: The cultivation of human and murine IL-2 dependent T-cell lines and natural killer cell lines.The proliferation of mitogen-activa...


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Application

Recombinant IL-2, human, allows: The cultivation of human and murine IL-2 dependent T-cell lines and natural killer cell lines.The proliferation of mitogen-activated T-lymphocytes and natural killer cells.The establishment of human and murine thymocyte, splenocyte, or peripheral blood lymphocyte (PBL) derived T-cell lines.The generation of human and murine lymphokine-activated killer (LAK) cells (1-11).In vitro re-stimulation of lung cells obtained from mice.

Biochem/physiol Actions

Interlekin-2 (IL-2) is a potent pro-inflammatory cytokine. It plays a protective role in autoimmune chronic inflammation, when present in low amounts. It is involved in maintaining immune homeostasis where it controls the cross-talk between T regulatory and T effector cells. Studies in acute myelogenous leukemia (AML) mice models show that antibody based delivery of IL-2 to targets present in tumor-linked vasculature results in antileukemic activity.

General description

Recombinant Interleukin-2, human (hIL-2) is produced in E. coli and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 µm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 µm pore size membrane

Preparation Note

Working concentration: 10 -20 U/mlEstablished IL-2-dependent T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependent cell lines.Working solution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1 to 10% serum.Storage conditions (working solution): -15 to -25 °CIt is recommended to store the solution in aliquots at -15 to -25 °C.Note: Avoid repeated freezing and thawing.Specific activity/EC 50 : >2 x 106 U/mg ﹤0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50 ) compared to the indicated standard is guaranteed (19, 20). Human, recombinant IL-2 has the same biological activity in vitro as compared to human, natural IL-2 (15, 1–4).Recommended Method of Dilution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1–10% serum.Interleukin-2 (IL-2, also known as T-Cell Growth Factor, TCGF) is a lymphokine which is produced by lectin- or antigen-activated T-cells and plays an important immunoregulatory role. This factor, or lymphokine, was first identified by its ability to promote the long-term in vitro proliferation of activated T cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine- activated killer (LAK) cells (1–11). Recombinant human IL-2 allows the cultivation of human and murine IL-2-dependent T-cell lines and natural killer cell lines, the proliferation of mitogenactivated T-lymphocytes and natural killer cells, the establishment of human and murine thymocyte-, splenocyte-, or peripheral blood lymphocyte (PBL)- derived T-cell lines, and the generation of human and murine lymphokine-activated killer (LAK) cells (1-11). Established IL-2-dependant T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependant cell lines.

Quality

Purity: >95% pure as determined by SDS-PAGEEndotoxin level: ﹤0.1 EU/µg (LAL-test), ﹤10 EU/ml (LAL-test)(1 EU corresponds to 0.1 ng)

Sequence

Chain Length 134 AA

The primary structure of recombinant, IL-2, human is identical to that of natural, human IL-2 (one polypeptide chain, 133 amino acids), however, recombinant IL-2 has an extra methionine at the amino-terminus (one polypeptide chain, 134 amino acids) and is not glycosylated. Glycosylation is not essential for biological activity.

Specificity

Species specificity: Recombinant IL-2, human is effective on mouse and human cells.

Unit Definition

EC50 definition: The amount of hIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng).Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)

assay95% (SDS-PAGE)
biological sourcehuman
concentration10-20U/ml
formsolution
Gene Informationhuman ... IL2(3558)
impurities﹤0.1 EU/µg
manufacturer/tradenameRoche
mol wt(15,000 Da)
packagingpkg of 10,000 U (10799068001 [5 µg, 50 ml]), pkg of 50,000 U (11147528001 [25 µg, 5 ml]), pkg of 10,000 U (11011456001 [5 µg, 1 ml])
potency>2 x 10^6 units/mg EC50
Quality Level100
recombinantexpressed in E. coli
storage conditionavoid repeated freeze/thaw cycles
storage temp.−20°C
UniProt accession no.P60568
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10799068001
Unit:EACH
List Price: £222.00
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11011456001
Unit:EACH
List Price: £223.00
Source:List Price
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