ROCHE Endoproteinase Glu-C (V8 Protease)from Staphylococcus aureus V8

Code: 10791156001 D2-231

Application

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

Biochem/physiol Actions

Endoproteina...


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£165.00 EACH
£198.00 inc. VAT

Application

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

Biochem/physiol Actions

Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.

General description

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: The enzyme has its maximal activity in presence of SH-reagentsWorking concentration: 1 to 5 mMWorking solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).Storage conditions (working solution): -15 to -25 °CThe enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.

Specificity

Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

formlyophilized (salt-free)
manufacturer/tradenameRoche
mol wt30 kDa
optimum pH8.0-8.5
packagingpkg of 2 mg
specific activity20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).), ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)
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