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Acetyl-Coenzyme A

Code: 10101907001 D2-231

Application

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl...


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Application

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.

Biochem/physiol Actions

Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.

General description

Acetyl-Coenzyme A, trilithium salt

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).Storage conditions (working solution): -15 to -25 °C50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.

assay85% (Enzymatic and Absorbance), 2% (lithium)
descriptionChemical Formula: C23H35N7O17P3SLi3
formsolid
manufacturer/tradenameRoche
mol wtMr 809.6 (acetyl-CoA), Mr 827.4 (acetyl-CoA-Li3)
packagingpkg of 050 mg (10101907001), pkg of 010 mg (10101893001), pkg of 200 mg (11585371001)
Quality Level100
storage temp.2-8°C
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10101893001
Unit:EACH
List Price: £99.50
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11585371001
Unit:EACH
List Price: £1,160.00
Source:List Price
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Cas Number75520-41-1
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