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Superoxide Dismutase from bovine liver, ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Code: S8409-15KU D2-231

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Application

Supero...


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Your Price
£160.00 EACH

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Application

Superoxide dismutase from bovine liver has been used in a study to assess the inactivation of endothelial derived relaxing factor by oxidized lipoproteins. Superoxide dismutase from bovine liver has also been used in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Superoxide Dismutase from bovine liver has been used:for measuring mitochondrial hydrogen peroxide production in skeletal muscleas a positive control in Cu(II) binding stability assayas a positive control in superoxide dismutase assay

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

General description

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Packaging

3000, 15000 units in glass bottle

Physical form

Suspension in 3.8 M (NH4)2SO4, pH 7.0

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial δA550 of 0.025 ± 0.005 per min.

formammonium sulfate suspension
Gene Informationcow ... SOD1(281495)
mol wt32.5 kDa
Quality Level200
specific activity2,000-6,000 units/mg protein (biuret)
storage temp.2-8°C
UniProt accession no.P00442
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Cas Number9054-89-1
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