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beta-Glucuronidase from Helix pomatia, Type H-1, partially purified powder, >=300,000 units/g solid

Code: G0751-1MU D2-231

Application

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydro...


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£352.00 EACH
£422.40 inc. VAT

Application

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

General description

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Packaging

100000, 500000, 1000000, 2000000 units in poly bottle

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

formpartially purified powder
Quality Level200
secondary activity≥10,000 units/g solid sulfatase
specific activity≥300,000 units/g solid
storage temp.−20°C
typeType H-1
Code
Description
Unit Size
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Cas Number9001-45-0
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