Analysis Note
ControlHuman lung tissue lysate
Application
Research CategorySignaling
Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Tara Hitke, Developmental Studies Hybridoma Bank, University of Iowa.)
Anti-Calcyclin Antibody, clone CPTC-Calcyclin-2 detects level of Calcyclin & has been published & validated for use in WB, ELISA, IH.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Calcyclin, also known as S100A6, is a calcium binding protein that is highly expressed in fibroblasts and epithelial cells. Inhibition of calcyclin by p53 is part of the mechanism to control cell proliferation. Calcyclin is associated with cancer and metastasis and plays a role in cell cycle regulation.
Immunogen
Full length recombinant protein corresponding to human Calcyclin.
Material obtained from the National Cancer Institute's Clinical Proteomic Technologies for Cancer (CPTC) program.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein G
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Calcyclin on 10 µg of human lung tissue lysate.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~10 kDa observed
This product has met the following criteria: