Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected retSDR1 immunoreactivity in human and rat retina tissue sections.
Immunofluorescence Analysis: Clone A11 hybridoma culture supernatant immunostained cone outer segments and a subset of inner nuclear layer somata in 4% paraformaldeyde-fixed, agarose-embedded bovine retina sections by fluorescent confocal microscopy (Courtesy of Francoise Haeseleer, Ph.D., University of Washington, Seattle, WA).
Western Blotting Analysis: A representative lot detected a greatly upregulated retSDR1 protein expression following p53 or TAp63γ overexpression induction in DLD-1 human colorectal adenocarcinoma cells (Kirschner, R.D., et al. (2010). Cell Cycle. 9(11):2177-2188).
Immunofluorescence Analysis: A representative lot immunostained cone, but not rod, outer segments in 4% paraformaldeyde-fixed, agarose-embedded bovine retina sections by fluorescent confocal microscopy. Immunoreactivity was also observed in a subset of somata localized in the inner nuclear layer (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799).
Immunohistochemistry Analysis: A representative lot immunostained cone, but not rod, outer segments in 4% paraformaldeyde-fixed bovine retina whole mount sections. Immunoreactivity was also observed in a subset of somata localized in the inner nuclear layer (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799).

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Anti-retSDR1 Antibody, clone A11 is an antibody against retSDR1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Short-chain dehydrogenase/reductase 3 (EC 1.1.1.300; UniProt O75911; also known as DD83.1, Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, retSDR1, Short chain dehydrogenase/reductase family 16C member 1) is encoded by the DHRS3 (also known as RDH17, SDR16C1) gene (ORF UNQ2424/PRO4983; Gene ID 9249) in human. The retSDR1 enzyme is an SDR (short-chain dehydrogenase/reductase) family member expressed in photoreceptor outer segments, where it mediates the reduction of all-trans-retinal for the regeneration of bleached pigments, the rate limiting step of retinoid reservoir replenishment during the visual cycle. Evidence indicates that retSDR1 also plays a role in tumor suppression. Alternation of vitamin A metabolism seems to be an important event in carcinogenesis and aberrant CpG-island methylation is reported in the DHRS3 promoter region in several neuroblastoma cell lines. The pro-apoptic p53 and p63 are both shown to regulate retSDR1 transcription.

Immunogen

Epitope: Within a.a. 169-303.

His-tagged recombinant full-length human retSDR1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting of overexpressed human retSDR1 in HEK293 transfectant lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected the expression of full-length human retSDR1 in 10 µg of lysate from transfected, but not untransfected HEK293 cells.

Specificity

Clone A11 recognized a truncated human retSDR1 comprising amino acid residues 169–303 by immunoblotting and immunostained cone, but not rod, outer segments by fluorescent confocal microscopy (Haeseleer, F., et al. (1998). J. Biol. Chem. 273(34):21790-21799). Epitope region is absent in human spliced isoform 2 (UniProt O75911-2).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~33 kDa observed. 33.55/33.50/33.61 kDa (human/bovine/rat) calculated. Uncharacterized band(s) may appear in some lysates.