Application

Anti-BDNF Antibody, clone 8G8.2 is an antibody against BDNF for use in Western Blotting.

General description

BDNF is a important neurotrophic factor essential for proper neuronal development and survival of various neuronal populations in the CNS and PNS. BDNF is also key for axonal growth and path finding of dendritic growth and morphology. BDNF also is key to neuronal responses and LTP or LTD and synaptic plasticity, behavior, learning and memory. BDNF is also important in neuronal disease and is being studied as potential marker in various diseases. Finally, BDNF is expressed initially as a proform protein long thought to simply be an inactive proform factor, however recent research demonstrates that proBDNF may play roles not only in neuronal development but also development in general as well as in adult metabolism in brain, muscle, and general metabolic function, so the research into BDNF is growing. BDNF is expressed developmentally in the brain and developing nervous system but it is also expressed in the adult in the brain, gut, muscle, lung, testis, prostate and placenta.

Immunogen

KLH-conjugated linear peptide corresponding to human BDNF.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in mouse brain membrane tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected BDNF in 10 µg of mouse brain membrane tissue lysate.

Target description

~25 kDa observed. UnitProt describes 5 isoforms produced by alternative promoter usage and alternative splicing ranging from 27.8 to 37.1 kDa