Application

This armenian hamster monoclonal Anti-FcgRIV (CD16-2) Antibody, clone 9E9, Cat. No. MABF853 is validated for use in Flow Cytometry and Neutralization.

General description

Transmembrane receptor CD16-2 (UniProt: Q8R477; also known as CD16-2, Fcgr4, FcgRIV, Fc gammaRIV, FcgammaRIV, Fcgr3a, CD16-2, Transmembrane receptor CD16-2) is encoded by the Fcgr4 (also known as Fcgr3a, Fcrl3) gene (Gene ID: 245256) in murine species. Murine species express three classes of activating IgG Fc receptors, the high affinity receptor FcgRI, the low affinity receptor FcgRIII, and the FcgRIV or CD16-2. CD16-2, a novel mouse receptor that displays significant homology to CD16/FcgRIII. It is a receptor for soluble IgG2a and 2b complexes, but not necessarily for cellular bound IgG2a. CD16-2 is expressed in peripheral blood leukocytes and in spleen, thymus, colon, and intestine. Similar to other activating receptors, CD16-2 possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM (immunoreceptor tyrosine-based activation motif) bearing accessory protein. Both CD16-2 (FcgRIV) and FcgRIII are considered to be essential for mediating type II and type III autoimmune responses via FcRc-LAT-dependent generation of C5a. However, FcgRIII-deficient mice display compensatory enhanced CD16-2 expression that offers protection from lung inflammation and exhibit reduced sensitivity to IgG2a/b-mediated hemolytic anemia. Ref.: Syed, S.N., et al (2009). Eur. J. Immunol. 39(12): 3343-3356; Mechetina, L.V., et al. (2002). Immunogenetics 54(7): 463-468.

Immunogen

Recombinant mouse FcgRIV extracellular domain fused to mouse IgG1 Fc fragment (Nimmerjahn, F., et al. (2005). Immunity. 23(1):41-51).

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified armenian hamster IgG in PBS without azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in RAW264.7 cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected FcgRIV on the surface of one million RAW264.7 murine macrophages.

Specificity

Clone 9E9 immunostained murine macrophages, neutrophils, dendritic cells, but not FcgRIII-expressing NK cells. Clone 9E9 also stained a FcgRIV-negative, B220-positive peripheral blood cell population (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).

Target description

28.39/28.37/28.38 kDa (UniProt Q3TC44/Q8R2R4/Q8R477) calculated.