Anti-FcgRII/III; clone Ab93

Code: MABF838 D2-231

Application

Flow Cytometry Analysis: A representative lot stained murine A20 B-cell lymphoma cells, but not the FcgammaRIl-deficient A20-IIA1.6 variant (Oliver, A.M., et al. ...


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Application

Flow Cytometry Analysis: A representative lot stained murine A20 B-cell lymphoma cells, but not the FcgammaRIl-deficient A20-IIA1.6 variant (Oliver, A.M., et al. (1999). Hybridoma 18(2):113-119).
Activity Assay Analysis: A representative lot prevented CD38-mediated proliferation of spleen-derived primary murine B-cells from wild-type, but not FcgammaRIl-deficient mice (Oliver, A.M., et al. (1999). Hybridoma 18(2):113-119).
Immunocytochemistry Analysis: A representative lot detected FcgRII association with cross-linkinked CD38 following anti-CD38 addition to cultured primary murine B-cells by fluorescent immunocytochemistry (Oliver, A.M., et al. (1999). Hybridoma 18(2):113-119).

This Anti-FcgRII/III Anibody, clone Ab93 is validated for use in Flow Cytometry, Immunocytochemistry, Activity Assay for the detection of FcgRII/II.

General description

Murine low affinity IgG Fc region receptor FcγRII and FcγRIII (UniProt P08101 and P08508, respectively) are encoded by the Fcgr2 (also known as Fcgr2b, Ly-17) and Fcgr3 gene, respectively. Four different classes of IgG Fc receptors (FcγRs) have been reported: FcγRI/CD32b, FcγRII/CD16, FcγRIII/CD16.2, and FcγRIV/CD64. FcγRII, FcγRIII, and FcγRIV (UniProt P08101, P08508, and Q8R477, respectively) are low-affinity receptors for monomeric IgG, whereas FcγRI (UniProt P26151) is the only high-affinity FcγR. FcγRs play important roles in inflammatory cell activation, clearance, presentation of antigen, and maintenance of IgG homeostasis. In addition to binding immune complex (IC), FcγRs have been shown to bind to non-IgG ligands. For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E. coli component negatively regulates the function of macrophage receptor with collagenous structure (MARCO). FcyRII activation is also reported to transduce a negative regulatory signal against CD38 cross-linking-induced proliferation of resting mature B cells.

Immunogen

Whole cells expressing mouse FcgRII/III.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Format: Purified

Quality

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 5.0 µg of this antibody detected FcgRII/III in mouse splenocytes.

Specificity

Recognizes both FcgammaRII and FcgammaRIII.

Target description

33.4 kDa (mature FcgRII) & 26.7 kDa (mature FcgRIII) calculated.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcerat
clone93, monoclonal
Gene Informationmouse ... Fcgr2b(14130), Fcgr3(14131)
isotypeIgG2aκ
NCBI accession no.NP_034317
Quality Level100
shipped inwet ice
species reactivitymouse
technique(s)immunocytochemistry: suitable, flow cytometry: suitable, activity assay: suitable
UniProt accession no.P08508, P08101
This product has met the following criteria: