Application

Western Blotting Analysis: A representative lot detected XRCC5 (Ku86) in Western Blotting applications (Knuth, M.W., et. al. (1990). J Biol Chem. 265(29):17911-20).

Research CategoryEpigenetics & Nuclear Function

Anti-XRCC5 (Ku86), clone N9C1, Cat. No. MABE1892, is a highly specific mouse monoclonal antibody that targets DNA repair protein XRCC5 and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

X-ray repair cross-complementing protein 5 (UniProt: P13010; also known as 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5) is encoded by the XRCC5 (also known as G22P2) gene (Gene ID: 7520) in human. XRCC5 is a single stranded DNA-dependent ATP-dependent helicase that exists as a heterodimer composed on XRCC5/XRCC6. This dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by about 100-fold. It plays a role in chromosome translocation. It binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It stabilizes broken DNA ends and bring them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ (Non-homologous end joining) ligation step. In association with NAA15, the XRCC5/6 dimer is shown to bind to the osteocalcin promoter and activates osteocalcin expression. The Ku domain of XRCC5 is localized in amino acids 251-460. It also contains an EEXXXDL motif (aa 720-728), which is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. It undergoes phosphorylation on serine residues by PRKDC that enhances its helicase activity.

Immunogen

Full-length human recombinant DNA repair protein XRCC5 (Ku86).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in SW480 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected XRCC5 (Ku86) in SW480 cell lysate.

Specificity

Clone N9C1 is a mouse monoclonal antibody that specifically detects DNA repair protein XRCC5.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~80 kDa observed; 82.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).