Application

Research CategoryEpigenetics & Nuclear Function

Anti-BMAL1 (ARNTL), clone B1BH2, Cat. No. MABE1886, is a highly specific mouse monoclonal antibody that targets Brain and muscle ARNT-like 1 (BMAL1) and has been tested for use in Chromatin Immunoprecipitation (ChIP) and Western Blotting.

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected BMAL1 (ARNTL) in Chromatin Immunoprecipitation applications (Yoshitane, H., et. al. (2014). Mol Cell Biol. 34(10):1776-87).

Western Blotting Analysis: A representative lot detected BMAL1 (ARNTL) in Western Blotting applications (Yoshitane, H., et. al. (2009). Mol Cell Biol. 29(13):3675-86; Yoshitane, H., et. al. (2012). EMBO Rep. 13(5):455-61).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: Q9I8T7; also known as BMAL1b′, Brain and muscle ARNT-like 1, cBMAL1) is encoded by the ARNTL (also known as BMAL1) gene (Gene ID: 374115) in chicken. BMAL1 is expressed in pineal gland and retina and displays maximum levels between ZT13 and ZT16 during light/dark cycle. BMAL1 is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is highly expressed in the adult brain, skeletal muscle, and heart. It shuttles between the nucleus and the cytoplasm and this nucleocytoplasmic shuttling is essential for the nuclear accumulation of CLOCK, target gene transcription, and the degradation of the CLOCK-ARNTL/BMAL1 heterodimer. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and premature aging.

Immunogen

MBP-conjugated recombinant fragment corresponding to 56 amino acids from the N-terminal region of chicken BMAL1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BMAL1 (ARNTL) in NIH/3T3 cell lysate.

Specificity

Clone B1BH2 specifically detects Brain and muscle ARNT-like 1 (BMAL1). It targets an epitope within 56 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed; 69.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).