Application

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A representative lot detected CSB (ERCC6) in Immunocytochemistry applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CSB (ERCC6) in Immunoprecipitation applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CSB (ERCC6) in Chromatin Immunoprecipitation applications (Kristensen, U., et. al. (2013). Proc Natl Acad Sci USA. 110(25):E2261-70; Velez-Cruz, R., et. al. (2012). Proc Natl Acad Sci USA. 110(3):E212-20).

Western Blotting Analysis: A representative lot detected CSB (ERCC6) in Western Blotting applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).

Anti-CSB (ERCC6), clone ICSB-3H8, Cat. No. MABE1831, is a highly specific mouse monoclonal antibody that targets DNA excision repair protein ERCC-6 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DNA excision repair protein ERCC-6 (UniProt: Q03468; also known as ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB) is encoded by the ERCC6 (also known as CSB) gene (Gene ID: 2074) in human. CSB is a homodimeric nuclear protein that belongs to the SwItch/ Sucrose nonfermentable family of DNA-dependent ATPases and contains seven characteristic helicase motifs. It is a part of a complex that contains RNA pol I, TFIIH, and XPG, all of which are active in transcription and/or DNA repair. Hence, it is an essential factor involved in transcription-coupled nucleotide excision repair, which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. It is shown to be essential for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the at sites of RNA polymerase II-blocking lesions. Mutations in CSB (ERCC6) gene are known to cause age related macular degeneration and Cockayne syndrome B that is characterized by delayed neural development and severe progressive neurologic degeneration and mental retardation.

Immunogen

Ovalbumin-conjugated linear Peptide corresponding to 22 amino acids from the C-terminal region of human DNA excision repair protein ERCC-6.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in A431 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CSB (ERCC6) in one million A431 cells.

Specificity

Clone 1CSB-3H8 specifically detects DNA excision repair protein ERCC-6 in human cells. It targets an epitope with in 22 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

168.42 kDa calculated.