MABE143 Display Image

Anti-Ago1; clone 4G7-E12

Code: MABE143 D2-231

Application

Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. N...


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Application

Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.

General description

Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

Immunogen

GST-tagged recombinant protein corresponding to human Ago1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.

Target description

~97 kDa observed.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone4G7-E12, monoclonal
Gene Informationhuman ... AGO1(26523)
isotypeIgG2aκ
NCBI accession no.NP_036331
Quality Level100
shipped inwet ice
species reactivityhuman
technique(s)western blot: suitable
UniProt accession no.Q9UL18
This product has met the following criteria: