Application

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

The unconjugated antibody (Cat. No. MAB318) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P07101; also known as Dystonia 14, TH, Tyrosine 3-hydroxylase) is encoded by the TH (also known as DYT14, DYT5b, TYH) gene (Gene ID 7054) in human. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of dopamine and norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six isoforms of human TH exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).

Immunogen

Tyrosine Hydroxylase purified from PC12 cells.

Epitope: On the outside of the regulatory N-terminus of tyrosine hydroxylase.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein A purified

Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Quality

Evaluated by Immunocytochemistry in rat cortical neurons.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tyrosine Hydroxylase in rat cortical neurons.

Specificity

Recognizes an epitope on the outside of the regulatory N-terminus. The unconjugated antibody (Cat. No. MAB318) recognizes a protein of approximately 59-61 kDa by Western blotting. The unconjugated antibody does not react with the following by Western blotting: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~59-63 kDa observed.