Analysis Note

ControlHeLa cells

Application

Research Sub CategoryEpitope Tags

Research CategorySecondary & Control Antibodies

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry.

Disclaimer

Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: CBL600

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Flow Cytometry in HeLa cells.
Flow Cytometry Analysis: 2 µg of this antibody was used as a Mouse IgG1 Negative Control in staining of HeLa cells.

Specificity

This isotype control shows negligible cross-reactivity with human cell surface antigens on tissue sections or in cellular preparations. It therefore enables the estimation of non-specific binding of mouse monoclonals to cell surface antigens.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.