Analysis Note

ControlHuman kidney tissue lysate

Application

Detect the NENF protein using this Anti-NENF validated for use in WB & IHC.

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Immunohistochemistry Analysis: 10 µg/mL of this antibody from a representative lot detected NENF in rat kidney tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

Immunogen

KLH-conjugated linear peptide corresponding to human NENF.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed