05-1003 Display Image

Anti-phospho-Akt (Ser473)

Code: 05-1003 D2-231

Application

Anti-phospho-Akt (Ser473) Antibody, clone 6F5 detects level of phospho-Akt (Ser473) & has been published & validated for use in ELISA, WB, FC, IF, IH.

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£399.00 EACH
£478.80 inc. VAT

Application

Anti-phospho-Akt (Ser473) Antibody, clone 6F5 detects level of phospho-Akt (Ser473) & has been published & validated for use in ELISA, WB, FC, IF, IH.

Immunofluroescence Analysis:
EGF-treated A431 cells were fixed, permeabilized, and stained with Anti-phospho-Akt (Ser473), clone 6F5 (2 µg/mL) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor™488 (green) was used to stain actin.

Immunohistochemistry:
Anti-phospho-Akt (Ser473), clone 6F5 was diluted to 1:200, IHC-Select Detection with HRP-DAB.

Flow Cytometry:
Anti-phospho-Akt (Ser473) was used at a 1:800 dilution for this assay.

Immunogen

Peptide encompassing and including phosphorylated Ser473 of human Akt1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monolconal IgG1κ in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05%NaN3

Quality

Western Blot Analysis:
0.5-1 µg/mL of this lot detected phosphorylated Akt in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.

Specificity

Expected to react with most species as sequence is 100% homologous with most common vertebrate.

Recognizes Akt only when phosphorylated on Ser473. This antibody does not cross-react with Akt1/PKBα when it is not phosphorylated on Ser473.

Target description

60 kDa

antibody formpurified antibody
antibody product typeprimary antibodies
biological sourcemouse
clone6F5, monoclonal
Gene Informationhuman ... AKT1(207)rat ... Akt1S1(292887)
isotypeIgG1κ
NCBI accession no.NP_001014431
Quality Level100
shipped inwet ice
species reactivityrat, mouse, human, vertebrates
technique(s)western blot: suitable, flow cytometry: suitable, ELISA: suitable, immunohistochemistry: suitable, immunofluorescence: suitable
UniProt accession no.P31749
This product has met the following criteria: