76307-100MG Display Image

Pefabloc SC, analytical standard

Code: 76307-100MG D2-231

Application

Recommended concentrations for use are 0.1-1 mM.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument techniqu...


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Your Price
£98.58 100MG
£118.30 inc. VAT

Application

Recommended concentrations for use are 0.1-1 mM.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Pefabloc® SC may be used as an analytical standard in determining the native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes. It may also be used as an esterase inhibitor, which is added to the whole blood to improve the stability of ciclesonide for the determination of ciclesonide (CIC) and its active metabolite, desisobutyryl-ciclesonide (des-CIC), in human plasma using liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric (LC-APCI-MS/MS) method.

Biochem/physiol Actions

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

General description

Pefabloc® SC is a potent, nontoxic and relatively easy to use, irreversible serine proteinase inhibitor. It is reported to efficiently inhibit the platelet activating factor (PAF)-degrading acetylhydrolase (acetylhydrolase) in both humans and rats.

Pefabloc® SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.

Legal Information

Pefabloc is a registered trademark of Pentapharm

Other Notes

Potent and irreversible inhibitor of serine proteases; in contrast to PMSF, it is water soluble and non-toxic; Unaffected by albumin

application(s)forensics and toxicologypharmaceutical (small molecule)
assay≥95.0% (HPLC)
formpowder or crystals
formatneat
gradeanalytical standard
InChI keyWRDABNWSWOHGMS-UHFFFAOYSA-N
InChI1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H
Quality Level200
shelf life5 yr
SMILES stringFS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
storage temp.2-8°C
technique(s)gas chromatography (GC): suitable, HPLC: suitable
Code
Description
Unit Size
List Price
Qty
76307-1G
Unit:1G
List Price: £661.89
Source:List Price
ADD
76307-500MG
Unit:500MG
List Price: £373.58
Source:List Price
ADD
Cas Number30827-99-7
This product has met the following criteria: