Analysis Note

www.gelifesciences.com.

Application

HiTrap^® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC^™ System

Features and Benefits

• For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.• Conveniently charge with your metal of choice.• Iminidoacetic acid coupled to Sepharose^® High Performance.• Purifying Proteins with exposed histidine groups via metal ion complex formation.• Simple operation with a syringe, pump or chromatographic system such as ÄKTA^® design or FPLC^™ System.• Packed with Chelating Sepharose^® High Performance• Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

General description

HiTrap^® Chelating HP is prepacked with Chelating Sepharose^® High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Legal Information

HiTrap is a registered trademark of GE Healthcare

ÄKTA is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

FPLC is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)