HITRAP HEPARIN HP 1 X 5 ML

Code: GE17-0407-01 D2-232

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• Heparin binds to...


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Your Price
£236.00 EACH
£283.20 inc. VAT

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.• Simple operation with a syringe, a pump or a chromatography system such as AKTA design• Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.

General description

HiTrap® Heparin HP columns are prepacked with Heparin Sepharose® High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.Heparin is coupled to the Sepharose® High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.

Legal Information

HiTrap is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

bed volume5 mL
avg. part. size34 µm
column I.D.16 mm
bed size16 mm × 25 mm
mfr. no.GE Healthcare, 17-0407-01
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packagingpack of 1 × 5 mL
parameter42 psi
parameter≤20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
working range5 - 10
cleaning5 - 10
Cas NumberMixture
This product has met the following criteria: