Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.• Simple operation with a syringe, a pump or a chromatography system such as AKTA design• Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.

General description

HiTrap^® Heparin HP columns are prepacked with Heparin Sepharose^® High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.Heparin is coupled to the Sepharose^® High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap^® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap^® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.

Legal Information

HiTrap is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)