Application

Yeast Nitrogen Base Without Amino Acids has been used:in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing and characterizationas a component of selective liquid media for uracil auxothrophic growth in yeastto evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

Components

Component, amount (µg/L unless indicated)Nitrogen Sources:Ammonium sulfate, 5.0 g/LVitamins:Biotin, 2.0Calcium pantothenate, 400Folic acid, 2.0Inositol, 2.0 mg/LNicotinic acid, 400p-Aminobenzoic acid, 200Pyridoxine HCl, 400Riboflavin, 200Thiamine HCL, 400Trace Elements:Boric acid, 500Copper sulfate, 40Potassium iodide, 100Ferric chloride, 200Manganese sulfate, 400Sodium molybdate, 200Zinc sulfate, 400Salts:Potassium phosphate monobasic, 1.0 g/LMagnesium sulfate, 0.5 g/LSodium chloride, 0.1 g/LCalcium chloride, 0.1 g/L

General description

Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Preparation Note

1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.2. Warm if necessary to solubilize and sterilize by filtration.Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.