Application

Triton^™-X has been used:In the permeabilization of cells for immunofluorescence stainingAs a component of lysis buffer in western blot analysisAs a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Packaging

5 mL in glass bottle

100, 500 mL in poly bottle

1 gal in poly bottle

1 L in poly bottle

5 gal in poly drum