Application

ThermaStop^™ has been used as a component of the PCR mix for the human immunodeficiency virus (HIV-1) proviral genome amplification by non-multiplexed nested PCR. It has also been used for the amplification of DNA from oral swabs of HIV infected individuals.

Features and Benefits

Adding ThermaStop^™ to your PCR reactions improves sensitivity and total product yield Increases the product yield for downstream applications like genotyping, next-generation sequencing (NGS) sample preparation, Gibson assembly, diagnostics, and multiplex PCR It improves polymerase specificity, enhances PCR performance and quantitative data Prevents primer-dimer formation and non-specific products before PCR Enables stabilization of master mixes at room temperature before PCR for up to 36 hrs Rapidly inactivated during the first PCR cycle Rapidly re-activated upon cooling

General description

ThermaStop^™ is a reversible hot-start reagent that is compatible with thermostable DNA polymerases. It acts directly on the polymerase to prevent non-specific enzymatic activity below 50°C. Polymerase activity is fully restored at 60°C, but is inhibited again by ThermaStop^™ by cooling the reaction. Adding ThermaStop^™ to your PCR reactions improves sensitivity and total product yield.

Legal Information

ThermaStop is a trademark of ThermaGenix Inc.

Reconstitution

Dissolve in Tris pH 8.3 buffer